Journal
BIOCONJUGATE CHEMISTRY
Volume 20, Issue 12, Pages 2364-2370Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc9003519
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Funding
- Swedish Research Council
- Royal Institute of Technology
- National Institutes of General Medical Science (NIGMS) [R01GM080295, 2R15GM066279]
- RL-ONAMI Center for Nanoarchitectures for Enhanced Performance
- European Commission [MRTN-CT-19561]
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Methods to rapidly functionalize specific polymeric surfaces with alkynes, which can subsequently be linked to azide-containing carbohydrates, are presented. The methods comprise two main concepts: azide photoligation and Cu-catalyzed azide-alkyne cycloaddition. 2-Azidoethyl-functionalized CL-D-mannopyranoside was synthesized and covalently attached to alkyne-functionalized polymeric surfaces using the techniques. The protein recognition properties of the carbollydrate-presenting surfaces were evaluated using quartz crystal microbalance biosensor instrumentation.
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