Journal
BIOCONJUGATE CHEMISTRY
Volume 20, Issue 9, Pages 1783-1791Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc9002037
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Funding
- Israel Science Foundation (ISF)
- Binational Science Foundation (BSF)
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The development and application of tools that allow labeling of proteins in vitro and in vivo is a highly active and interdisciplinary area of research. Self-immolative polymers (SIPs) are a unique type of molecules that respond to external stimuli by undergoing head-to-tail disassembly through a self-immolative fragmentation. We have demonstrated how these polymers can be applied as activity-linked labeling probes for proteins with catalytic activity. A real-time detection of the protein labeling can be monitored due to a change in the fluorescence emission wavelength of the probe during the polymer disassembly process. ne SIP-based labeling approach showed considerable conservation of catalytic activity of the labeled protein and significant preference toward labeling of the activating protein.
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