Journal
BIOCONJUGATE CHEMISTRY
Volume 20, Issue 8, Pages 1569-1577Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc9001147
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Funding
- Canada Research Chair on Protein-Enhanced Biomaterials
- Canada Research Chair in Applied Metabolic Engineering
- Natural Sciences and Engineering Research Council of Canada
- GRSTB
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We have elaborated and validated a novel approach for the oriented tethering of proteins such as the epidermal growth factor (EGF) oil aminated surfaces. The grafting reactions were optimized to generate a dense and homogeneous EGF layer. Impact of EGF orientation on A-431 cellular response was investigated. Our results demonstrate that, in sharp contrast to responses obtained with soluble EGF supply or with randomly grafted EGF, oriented immobilization of EGF via a de novo designed coiled-coil capture system leads to a sustained phosphorylation of A-431 cell surface EGF receptors. Our results thus indicate that oriented protein immobilization via coiled-coil interactions is an efficient: and versatile method to control tethering of bioactive molecules for future applications in the field of regenerative medicine and tissue engineering.
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