Journal
ANALYST
Volume 140, Issue 16, Pages 5570-5577Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an00704f
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Funding
- NSFC [21475126]
- Jilin Provincial Science and Technology Department [20100701]
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It is important to develop methods to determine cylindrospermopsin (CYN) at trace levels since CYN is a kind of widespread cyanobacterial toxin in water sources. In this study, a label-free impedimetric aptasensor has been fabricated for detecting CYN. In this case, the amino-substituted aptamer of CYN was co-valently grafted onto the surface of the thionine-graphene (TH-G) nanocomposite through the crosslinker glutaraldehyde (GA). The reaction of the aptamer with CYN was monitored by electrochemical impedance spectroscopy because the CYN induced conformation change of the aptamer can cause a remarkable decrease of the electron transfer resistance. Under optimum conditions, the aptasensor exhibits high sensitivity and a low detection limit for CYN determination. The CYN can be quantified in a wide range of 0.39 to 78 ng mL(-1) with a good linearity (R-2 = 0.9968) and a low detection limit of 0.117 ng mL(-1). In addition, the proposed aptasensor displays excellent stability, reusability and reproducibility.
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