Journal
AIDS
Volume 14, Issue 18, Pages 2841-2849Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00002030-200012220-00005
Keywords
neurological/brain; magnetic resonance spectroscopy; immunohistochemistry; primate; SIV; pathogenesis
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Funding
- NCRR NIH HHS [RR13213] Funding Source: Medline
- NINDS NIH HHS [NS34626, NS30769] Funding Source: Medline
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Objective: To specify the type and severity of cellular damage in the central nervous system soon after infection and at later stages of disease in the SIV-macaque model of AIDS. Design and methods: Adjacent samples of frontal cortical gray matter were taken from three groups of macaques: uninfected controls (n = 4), acute (14 days post-infection; n = 4), and chronic (mean 2 years post-infection; n = 7). In vitro high resolution magnetic resonance spectroscopy of snap frozen intact tissue and quantitative neuropathology measurements of synaptophysin, calbindin, and glial fibrillary acidic protein (GFAP) in formalin-fixed tissue were performed. Results: Losses in n-acetylaspartate and calbindin (indicating neuronal injury and/or death) and decreases in synaptophysin immunoreactivity (indicating synaptodendritic injury) were detected along with increases in GFAP (indicating reactive gliosis). Cellular injury worsened progressively with increased time after infection. Conclusions: These results are the first direct evidence that neuronal injury occurs soon after infection. The exacerbation of injury with time suggests a connection between the early response of the central nervous system and dementia, which occurs fate in the course of infection. This connection may have broad implications for the study of and the development of therapies for damage of the central nervous system by (C) 2000 Lippincott Williams & Wilkins.
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