Genomic exploration of the hemiascomycetous yeasts: 2. Data generation and processing

The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE, These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.