Journal
FEBS LETTERS
Volume 487, Issue 1, Pages 13-16Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02273-0
Keywords
high-throughput sequencing; sequence analysis; genome; hemiascomycete
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The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE, These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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