Journal
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
Volume 34, Issue 2, Pages 414-426Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2015.1038585
Keywords
terbium(III) complex; 2-2 '-bipyridine; DNA-binding; thermodynamic parameters; spectroscopy; groove binding
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Funding
- University of Sistan and Baluchestan (USB)
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Agarose gel electrophoresis, absorption, fluorescence, viscosity, and circular dichroism (CD) have been used in exploring the interaction of terbium(III) complex, [Tb(bpy)(2)Cl-3(OH2)] where bipy is 2,2-bipyridine, with Fish salmon DNA. Agarose gel electrophoresis assay, along with absorption and fluorescence studies, reveal interaction between the corresponding complex and FS-DNA. Also, the binding constants (K-b) and the Stern-Volmer quenching constants (K-sv) of Tb(III) complex with FS-DNA were determined. The calculated thermodynamic parameters suggested that the binding of mentioned complex to FS-DNA was driven mainly by hydrophobic interactions. A comparative study of this complex with respect to the effect of iodide-induced quenching, ionic strength effect, and ethidium bromide exclusion assay reflects binding of explicit to the FS-DNA primarily in a groove fashion. CD and viscosity data also support the groove binding mode. Furthermore, Tb(III) complex have been simultaneously screened for their antibacterial and antifungal activities.
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