Journal
BIOCHIP JOURNAL
Volume 7, Issue 3, Pages 234-241Publisher
KOREAN BIOCHIP SOCIETY-KBCS
DOI: 10.1007/s13206-013-7306-5
Keywords
Bacterial cellulose; Gold nanoparticles; Hydrogel; Three-dimensional substrate; Surface-enhanced Raman spectroscopy; SERS hot spot
Funding
- National Research Foundation of Korea (NRF)
- Ministry of Science, ICT & Future Planning [2013023612, 2013-006163]
- Pioneer Research Center Program through the National Research Foundation of Korea
- Research Institute for Agriculture and Life Sciences
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Bacterial cellulose hydrogels containing gold nanoparticles (AuNPs-BC) were prepared using a cost-effective and environment-friendly in situ synthesis method. Well-dispersed AuNPs were grown on the nanofiber surface of the BC hydrogel, forming substrates of surface-enhanced Raman spectroscopy (SERS). Increases in SERS-active sites in the AuNPs-BC hydrogel caused the enhancement of SERS intensity. The enhancement in SERS intensity of 4-fluorobenzenethiol and phenylacetic acid (PAA), used as test analytes, was compared with spatially-un-deformed AuNPs-BC hydrogels, as well as spatially-deformed AuNPs-BC hydrogels in which the BC layers had contracted during drying. Particularly noteworthy was the detection of PAA by the simple contraction of the substrate, despite a low affinity to surface gold.
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