Journal
FEBS LETTERS
Volume 487, Issue 3, Pages 347-350Publisher
WILEY
DOI: 10.1016/S0014-5793(00)02342-5
Keywords
chloroplast nucleoid; DNA-binding protein; sulfite reductase; Pisum sativum
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The chloroplast nucleoid is a complex of chloroplast DNA and various, mostly uncharacterized proteins. An abundant 70-kDa protein of the isolated nucleoids of pea chloroplasts was identified as sulfite reductase by N-terminal sequence analysis as well as immunoblot analysis, spectrophotometry and enzyme activity analysis. Recombinant maize sulfite reductase was indeed able to compact chloroplast DNA and to form nucleoid-like particles in vitro. The role of sulfite reductase in the structural organization of the nucleoid is discussed. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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