Journal
VACCINE
Volume 19, Issue 11-12, Pages 1456-1459Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0264-410X(00)00350-9
Keywords
OPV; Vero cell; MAPREC
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To confirm the safety of oral poliomyelitis vaccine (OPV) cultured in Vero cells. the genetic stability of cultured polio vaccine viruses was analysed by MAPREC (mutant analysis by PCR and restriction enzyme cleavage). The rates of mutant accumulation of the viruses passaged in Vero cells under a low multiplicity of infection (MOI) condition (approximately 10 (-) (3.5) CCID50/cell; the same as under usual OPV production conditions) were higher than those passaged in secondary cultured monkey kidney cells. However, the rates of mutant accumulation were restrained when the viruses were cultured under a high MOI condition (approximately 10 (-1.5) CCID50/cell) in Vero cells. Furthermore, neurovirulence of the passaged viruses in pollovirus susceptible transgenic mice PVR-Tg21 was shown to correlate highly with the results of MAPREC. It is expected that our results will contribute to the large scale preparation of safe and effective OPV using Vero cells. (C) 2001 Elsevier Science Ltd. All rights reserved.
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