Journal
BIOCHIMIE
Volume 101, Issue -, Pages 248-251Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2014.01.013
Keywords
IL-32; Yeast two-hybrid; Immunoprecipitation; Isoform interaction; Interaction map
Categories
Funding
- basic program of the National Research Foundation of Korea (NRF) [2012R1A2A2A 02008751, 2013-A423-0061]
- Priority Research Centres Program [2012-0006686]
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IL-32 has been studied for its pleiotropic effects ranging from host immune responses to cell differentiation. Although several IL-32 isoforms have been characterized for their effects on cells, the roles of the others remain unclear. We previously reported that IL-32 delta interacted with IL-32 beta and inhibited IL-32 beta-mediated IL-10 production. Thus, we performed comprehensive analyses to reveal more interactions between IL-32 isoforms in this study. We screened the interactions of 81 combinations of nine IL-32 isoforms by using a yeast two-hybrid assay, which identified 13 heterodimeric interactions. We verified these results by using reciprocal immunoprecipitation assays and reconfirmed 10 interactions, and presented the interaction network map between IL-32 isoforms. Our data suggest that IL-32 may have diverse intracellular effects through the interactions with its different isoforms. (C) 2014 Elsevier Masson SAS. All rights reserved.
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