Journal
NEUROSCIENCE LETTERS
Volume 297, Issue 2, Pages 97-100Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/S0304-3940(00)01675-X
Keywords
CD10; enkephalinase; common-acute lymphoblastic leukemia antigen; hippocampus; reverse transcription-polymerase chain reaction; mRNA; Western blots; microtubule-associated protein-2
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Neprilysin is an enzyme capable of degrading P-amyloid protein. We measured neprilysin mRNA and protein levels in brain and peripheral organs of Alzheimer disease (AD) and control cases. Neprilysin mRNA levels were lowest in the hippocampus and temporal gyrus, which are vulnerable to senile plaque development. They were highest in the caudate and peripheral organs which are resistant to senile plaque development. Levels in AD were significantly lower than controls in the hippocampus and midtemporal gyrus but not in other brain areas or peripheral organs. We a Iso measured levels of the mRNA for the neuronal marker microtubule-associated protein-2. They were remarkably constant in all brain areas and were not lowered in AD, indicating that the neprilysin mRNA reduction in the hippocampus and temporal gyrus was not correlated with simple neuronal loss. Relative levels of neprilysin protein generally paralleled those of the mRNA. These results suggest that deficient degradation of beta -amyloid protein caused by low levels of neprilysin may contribute to AD pathogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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