Journal
NUCLEIC ACIDS RESEARCH
Volume 29, Issue 2, Pages 573-577Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/29.2.573
Keywords
-
Categories
Funding
- NCI NIH HHS [T32 CA009139, CA16519, P01 CA016519, 5T32CA09139-24] Funding Source: Medline
- NIAID NIH HHS [R01 AI040227, AI40227, R37 AI040227] Funding Source: Medline
Ask authors/readers for more resources
L1 elements are human transposons which replicate via an RNA intermediate. At least 15% of the human genome is composed of L1 sequence. An important initial step in the transposition reaction is nicking of the genomic DNA by L1 endonuclease (L1 EN), In vivo much of the genome exists in the form of chromatin or is undergoing biochemical transactions such as transcription, replication or repair, which may alter the accessibility of the L1 transposition machinery to DNA, To investigate this possibility we have examined the effect of substrate chromatinization on the ability of L1 EN to nick DNA, We find that DNA incorporated into nucleosomes is generally refractory to nicking by L1 EN, Interestingly, nicking of a minority of DNA sequences is enhanced when included in chromatin, Thus, dynamic epigenetic factors such as chromatinization are likely to influence the relatively permanent placement of L1 and other retroelements in the human genome.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available