Journal
BIOCHIMIE
Volume 95, Issue 1, Pages 3-11Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2012.09.033
Keywords
Ezrin; Radixin and moesin; Biomimetic membranes; Phosphoinositol (4,5)-bisphosphate (PIP2); Large unilamellar vesicles; Supported lipid bilayers; Giant unilamelar vesicles
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Funding
- ANR PCV
- European Commission (FP7) via an ERC Starting Grant [GA259370]
- Association pour la Recherche contre le Cancer (ARC)
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Ezrin, radixin and moesin (ERM) proteins are more and more recognized to play a key role in a large number of important physiological processes such as morphogenesis, cancer metastasis and virus infection. Recent reviews extensively discuss their biological functions [1-4]. In this review, we will first remind the main features of this family of proteins, which are known as linkers and regulators of plasma membrane/cytoskeleton linkage. We will then briefly review their implication in pathological processes such as cancer and viral infection. In a second part, we will focus on biochemical and biophysical approaches to study ERM interaction with lipid membranes and conformational change in well-defined environments. In vitro studies using biomimetic lipid membranes, especially large unilamellar vesicles (LUVs), giant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs) and recombinant proteins help to understand the molecular mechanism of conformational activation of ERM proteins. These tools are aimed to decorticate the different steps of the interaction, to simplify the experiments performed in vivo in much more complex biological environments. (c) 2012 Elsevier Masson SAS. All rights reserved.
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