Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 276, Issue 3, Pages 1998-2006Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M005101200
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- NIGMS NIH HHS [GM 49345] Funding Source: Medline
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p53 represses the transcription of cdc2 and cyclin B1, causing loss of Cdc2 activity and Cf, arrest. Here we show that the region -22 to -2 of the cdc2 promoter called the R box is required for repression by p53 but not for basal promoter activity. The R box confers p53-dependent repression on heterologous promoters and binds to p130/E2F4 in response to overexpression of p53. R box-dependent repression requires p21/waf1, and overexpression of p21/waf1 also represses the cdc2 promoter. These observations suggest that p53 represses the cdc2 promoter by inducing p21/waf1, which inhibits cyclin dependent kinase activity, enhancing the binding of p130 and E2F4, which together bind to and repress the cdc2 promoter.
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