Journal
NEUROSCIENCE LETTERS
Volume 297, Issue 3, Pages 175-178Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(00)01691-8
Keywords
cannabinoids; 2-arachidonoylglycerol; anandamide; monoacylglycerol; decapitation; brain ischemia; rat
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Rat brain, frozen in liquid nitrogen immediately after decapitation, contains a substantial amount of 2-arachidonoylglycerol (0.34 nmol/g tissue), an endogenous cannabinoid receptor ligand. The level of 2-arachidonoylglycerol in the brain was rapidly augmented after decapitation, the peak being noted 30 s after decapitation (1.54 nmol/g tissue). Noticeably, there are two phases during the increase in the levels of 2-arachidonoylglycerol: a rapid transient increase and a subsequent gradual sustained increase, suggesting that at least two separate mechanisms are involved in the generation of 2-arachidonoylglycerol in the decapitated brain. Gradual sustained formation was also observed for other monoacylglycerols, (e,g, 2-paimitoylglycerol plus 2-oleoylglycerol and 2-cis-vaccenoylglycerol). Thus, it is important to minimize post-mortem changes to estimate the exact tissue levels of 2-arachidonoylglycerol as well as other monoacylglycerols in the brain. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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