4.6 Article

Hypoxia and nitric oxide induce a rapid, reversible cell cycle arrest of the drosophila syncytial divisions

Journal

JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 276, Issue 3, Pages 1930-1937

Publisher

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M003911200

Keywords

-

Funding

  1. NIGMS NIH HHS [R01 GM037193, R01 GM060988-01, GM 60988, R37 GM037193, R01 GM060988, R01 GM060988-02, GM 37193, R01 GM037193-14] Funding Source: Medline

Ask authors/readers for more resources

Cells can respond to reductions in oxygen (hypoxia) by metabolic adaptations, quiescence or cell death (1), The nuclear division cycles of syncytial stage Drosophila melanogaster embryos reversibly arrest upon hypoxia, We examined this rapid arrest in real time using a fusion of green fluorescent protein and histone 2A In addition to an interphase arrest, mitosis was specifically blocked in metaphase, much like a checkpoint arrest. Nitric oxide, recently proposed as a hypoxia signal in Drosophila, induced a reversible arrest of the nuclear divisions comparable with that induced by hypoxia. Syncytial stage embryos die during prolonged hypoxia, whereas post-gastrulation embryos (cellularized) survive (2, 3). We examined ATP levels and morphology of syncytial and cellularized embryos arrested by hypoxia, nitric oxide, or cyanide. Upon oxygen deprivation, the ATP levels declined only slightly in cellularized embryos and more substantially in syncytial embryos. Reversal of hypoxia restored ATP levels and relieved the cell cycle and developmental arrests. However, morphological abnormalities suggested that syncytial embryos suffered irreversible disruption of developmental programs. Our results suggest that nitric oxide plays a role in the response of the syncytial embryo to hypoxia but that it is not the sole mediator of these responses.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available