Journal
SCIENCE
Volume 291, Issue 5504, Pages 653-656Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1057661
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Funding
- NIGMS NIH HHS [GM47866, GM56312, GM53678] Funding Source: Medline
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The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclear-mitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-beta. We show that importin-beta is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-beta and NuMA, Importin-beta therefore Links NUMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis.
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