Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 305, Issue 4, Pages 689-702Publisher
ACADEMIC PRESS LTD
DOI: 10.1006/jmbi.2000.4328
Keywords
ppGpp; RNA polymerase; amino acid biosynthesis; stringent response; ribosomal RNA transcription
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Funding
- NIGMS NIH HHS [GM37048] Funding Source: Medline
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Strains containing ppGpp, a nucleotide whose synthesis is dependent on the RelA and SpoT proteins of Eschericlzia coil, display slightly lower rRNA promoter activity and much higher amino acid biosynthesis/transport promoter activity than Delta rel Delta spoT strains. In the accompanying paper, we show that ppGpp directly inhibits rRNA promoter activity in vitro by decreasing the lifetime of the a rrn P1 open complex. However, ppGpp does not stimulate amino acid promoter activity in vitro. We show here that RNA polymerase (RNAP) mutants, selected to confer prototrophy to al Delta rel Delta spoT strains, mimic the effects of ppGpp on wild-type RNAP. Based on the positions of the mutant residues that confer prototrophy in the structure of core RNAP, we suggest molecular models for how the mutants, and by analogy ppGpp, generally decrease the lifetime of open complexes. We show that amino acid promoters require higher concentrations of RNAP for function in vitro and in vivo than control promoters, and are more sensitive to competition for RNAP in vivo than control promoters. Furthermore, we show that the requirement of an amino acid promoter for ppGpp in vivo can be alleviated by increasing its rate-limiting RNAP-binding step. Our data are consistent with a previously proposed passive model in which ppGpp inhibits stable RNA synthesis directly by reducing the Lifetime of the rrn P1 open complex, Liberating enough RNAP to stimulate transcription from amino acid promoters. Our data also place considerable constraints on models invoking hypothetical factors that might increase amino acid promoter activity in a ppGpp-dependent fashion. (C) 2001 Academic Press.
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