Journal
BIOCHIMIE
Volume 92, Issue 2, Pages 187-193Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2009.11.008
Keywords
Broad-spectrum resistance; Jasmonates; Jasmonate-regulated proteins (JRP); Monocot jacalin-related lectins; Triticum aestivum L
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Funding
- National Natural Science Foundation of China [30671043, 30671134, 30970261]
- Natural Science Foundation of Beijing [6082018]
- National High Technology and Research Development Program of P. R. China [2007AA10Z101]
- Chinese National Special Foundation for Transgenic Plant Research and Commercialization [2008ZX08002-003]
- Innovation Project of Chinese Academy of Sciences
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Jasmonates are known to induce the transcriptional activation of plant defense genes, which leads to the production of jasmonate-regulated proteins (JRP). We previously cloned and characterized a novel jacalin-like lectin gene (Ta-JA1) from wheat (Triticum aestivum L.), which codes a modular JRP with disease response and jacalin-related lectin (JRL) domains and is present only in the Gramineae family. The function of this protein is still unclear. Phylogenetic analysis indicated that Ta-JA1 and related proteins from cereals grouped together, which diverged from JRL with an additional N-terminal disease response domain. The recombinant Ta-JA1 proteins agglutinated rabbit erythrocytes, and this hemagglutination activity was preferentially inhibited by mannose. The Ta-JA1 protein was able to inhibit E coli cell growth. Overexpression of Ta-JA1 in transgenic tobacco plants increased their resistance to infection by tobacco bacterial, fungal and viral pathogens. Our results suggest that Ta-JA1 belongs to a mannose-specific lectin, which may confer a basal but broad-spectrum resistance to plant pathogens. Ta-JA1 and its homologues in maize, rice, sorghum and creeping bentgrass may represent a new type of monocot lectin with a modular structure and diversity of physiological functions in biotic and abiotic stress responses. (C) 2009 Elsevier Masson SAS. All rights reserved.
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