Journal
TISSUE ANTIGENS
Volume 57, Issue 2, Pages 110-117Publisher
MUNKSGAARD INT PUBL LTD
DOI: 10.1034/j.1399-0039.2001.057002110.x
Keywords
HLA class I; neuroblastoma; TAP; tapasin; interferon-gamma
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We studied the constitutive and the interferon (IFN)-gamma -induced expression of HLA class I antigen heavy chain, beta (2)-microglobulin (beta (2)m), TAP-1, TAP-2 and tapasin in a panel of eleven neuroblastoma cell lines. Surface expression of HLA class I antigens was low in eight out of eight neuroblastoma cell lines bearing MYC-N amplification and/or Ip deletion, while two out of three neuroblastoma cell lines lacking these genetic alterations showed normal expression. IFN-gamma treatment restored HLA class I antigen surface expression in all neuroblastoma cell lines. Eight out of 11 neuroblastoma cell lines did not express TAP-1 mRNA and three of them also lacked TAP-2 mRNA. beta (2) m mRNA was barely detectable or absent in five neuroblastoma cell lines, while tapasin mRNA was always expressed. IFN-gamma upregulated the expression of HLA class I heavy chain, beta (2) m, TAP-1, TAP-2 and tapasin, as detected at mRNA or protein level. Post-transcriptional events were involved in altered TAP-1 and beta (2) m expression in one peculiar neuroblastoma cell line. These data indicate that multiple mechanisms play a role in the HLA class I antigen-deficient phenotype of human neuroblastoma.
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