Journal
BIOCHIMIE
Volume 90, Issue 7, Pages 1074-1087Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2008.01.005
Keywords
electrospray mass spectrometry; ligand; nucleic acid; non-covalent complex; binding constant
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We present here a tutorial review on the electrospray mass spectrometry technique and its applications to the study of drug-nucleic acid noncovalent complexes. Particular emphasis has been made on the basic principles of the technique, to allow even the non-specialist to design fit-forpurpose mass spectrometry experiments and interpret the results. Standard applications will be described in detail, including the determination of stoichiometries and equilibrium binding constants of non-covalent complexes, the study of binding kinetics, and the development of ligand screening assays. We also outline the potentials of more advanced and/or more recent MS-based techniques (tandem mass spectrometry, ion mobility spectrometry and gas-phase spectroscopy) for the study of the nucleic acid-ligand complexes. (c) 2008 Elsevier Masson SAS. All rights reserved.
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