Journal
INFECTION AND IMMUNITY
Volume 69, Issue 2, Pages 1127-1133Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.69.2.1127-1133.2001
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Funding
- NHLBI NIH HHS [HL55967, R01 HL055967] Funding Source: Medline
- NIAID NIH HHS [AI-44072, R01 AI044072] Funding Source: Medline
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Lung dendritic cells were identified by immunohistochemistry in lung tissue sections from C57BL/6 mice, Following isolation from the lungs using CD11c magnetic beads, the flow cytometric analysis of I-A(b+) and CD11c(+) cells indicated a mixed population of dendritic cells at different stages of maturation, with most expressing an immature phenotype, When cultured for 7 days with recombinant murine granulocyte-macrophage colony-stimulating factor, 99% of cells were CD11c(+) and had a morphology typical of immature dendritic cells. These cells were negative for CD34, CD14, and CD8 alpha antigens but expressed low levels of the myeloid marker F4/80 and moderate levels of MAC3, All expressed high levels of CD11a (LFA-1), CD11b (Mac1), and CD54 antigens, with low levels of class II major histocompatibility complex. Most cells expressed CD80 but only a small percentage of cells were positive for CD40 and CD86. Both overnight and 7-day cultures of lung dendritic cells were able to phagocytose Mycobacterium tuberculosis, and this was associated with the production of interleukin-la and stimulation of both naive and immune T cells to produce gamma interferon.
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