Journal
COMPARATIVE AND FUNCTIONAL GENOMICS
Volume 2, Issue 1, Pages 22-24Publisher
HINDAWI LTD
DOI: 10.1002/cfg.69
Keywords
DNA arrays; environmental marker cassette; functional analysis; gene expression; reporter genes; rhizosphere; signature-tagged mutagenesis
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Bacillus subtilis is a sporulating Gram-positive bacterium that lives primarily in the soil and associated water sources. Whilst this bacterium has been studied extensively in the laboratory, relatively few studies have been undertaken to study its activity in natural environments. The publication of the B. subtilis genome sequence and subsequent systematic functional analysis programme have provided an opportunity to develop tools for analysing the role and expression of Bacillus genes in situ. In this paper we discuss analytical approaches that are being developed to relate genes to function in environments such as the rhizosphere. Copyright (C) 2001 John Wiley & Sons, Ltd.
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