Journal
HYPERTENSION
Volume 37, Issue 2, Pages 599-603Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/01.HYP.37.2.599
Keywords
indomethacin; microcirculation; arterioles; potassium channels; cyclooxygenase
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Funding
- NHLBI NIH HHS [HL-51971] Funding Source: Medline
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Indomethacin or glibenclamide treatments attenuate functional dilation of larger-diameter feed'' arterioles paired with venules in hamster cremaster muscle. We tested the hypothesis that release of cyclooxygenase products from venules is important for functional dilation of third- and fourth-order arterioles. We also tested whether ATP-sensitive potassium channels are important during functional dilation of smaller arterioles. The microcirculation of hamster cremaster muscle was visualized with in vivo video microscopy. We measured diameter responses of third- and fourth-order arterioles paired and unpaired with venules in response to 2 minutes of muscle field stimulation (40 mus, 10 V, 1 Hz), Control diameters of vessels were 31+/-2 (n=19), 13+/-1 (n=12), 12+/-2 (n=12), and 10+/-1 (n=12) for paired and unpaired third-order and paired and unpaired fourth-order arterioles, respectively. In all groups, field stimulation resulted in increases in mean control diameter of >80%. Indomethacin (28 mu mol/L) superfused on the preparation was used to inhibit cyclooxygenase metabolism, or glibenclamide (10 mu mol/L) was used to block ATP-sensitive potassium channels. Indomethacin attenuated arteriolar vasodilations to electrical stimulation in paired third-order vessels only, whereas glibenclamide attenuated this vasodilation in all 4 groups. These results support a role for ATP-sensitive potassium channels in functional dilation of arterioles of all sizes regardless of whether or not they are paired with venules, Conversely, a role for cyclooxygenase products is limited to larger feed arterioles paired with venules. This study provides further evidence that venules may be the source of prostaglandin release during functional hyperemia.
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