Journal
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
Volume 1834, Issue 6, Pages 1239-1256Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2012.11.013
Keywords
Native mass spectrometry; Nanoelectrospray ionization; Ion mobility; Covalent labeling; H/D exchange; Cross-linking
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Mass spectrometry-based methods have become increasingly important in structural biology - in particular for large and dynamic, even heterogeneous assemblies of biomolecules. Native electrospray ionization coupled to ion mobility-mass spectrometry provides access to stoichiometry, size and architecture of noncovalent assemblies; while non-native approaches such as covalent labeling and H/D exchange can highlight dynamic details of protein structures and capture intermediate states. In this overview article we will describe these methods and highlight some recent applications for proteins and protein complexes, with particular emphasis on native MS analysis. This article is part of a Special Issue entitled: Mass spectrometry in structural biology. (C) 2012 Elsevier B.V. All rights reserved.
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