Effect of osmotic pressure on paclitaxel production in suspension cell cultures of Taxus chinensis

The effect of osmotic pressure on paclitaxel production was investigated in the suspension cell cultures of Taxus chinensis. Paclitaxel production was definitely influenced by the initial sucrose concentration and the highest production yield was achieved at the concentration of 60 g.l(-1) sucrose (300 mOsm.kg(-1)). High osmotic pressure conditions generated by non-metabolic sugar (mannitol and sorbitol) also enhanced paclitaxel production by about two-fold. Kinetic studies revealed that high initial osmotic pressure enhanced paclitaxel production and that high concentration of sucrose was effective for sustaining secondary metabolism after induction of paclitaxel biosynthesis. Stoichiometric analysis with different combinations of sucrose and mannitol confirmed that osmotic pressure was the more important factor for enhancing paclitaxel metabolism. The addition of non-sugar osmotic agent, PEG also enhanced paclitaxel production. In this paper, we showed that high osmotic pressure led to increases in paclitaxel production and proposed that regulation of osmotic pressure may be useful in controlling paclitaxel production. (C) 2001 Elsevier Science Inc. All rights reserved.