Journal
EXPERIMENTAL CELL RESEARCH
Volume 263, Issue 1, Pages 163-172Publisher
ELSEVIER INC
DOI: 10.1006/excr.2000.5113
Keywords
RAR; RXR; retinoids; tight junctions; F9 cells
Categories
Ask authors/readers for more resources
Retinoids are critical for differentiation of columnar epithelial cells and for preventing metaplasia of these cells into stratified squamous epithelial cells, in which tight junctions (TJs) are essentially absent. This implies that retinoids might play important roles in regulating the structures and functions of TJs of columnar epithelium. F9 murine embryonal carcinoma cells differentiate into epithelial cells resembling visceral endoderm bearing TJs, when grown in suspension as aggregates in the presence of retinoic acid (RA). We show that RA induces the TJ structure and expression of several TJ-associated molecules, such as ZO-1, occludin, claudin-6, and claudin-7, as well as a barrier function in the genetically engineered cell line F9: rtTA:Cre-ERT L32T2, which allows sophisticated genetic manipulations simply by addition of ligands (H. Chiba et at, 2000, Exp. Cell Res. 260, 334-339). Interestingly, our data indicate that a barrier for small substances is generated after that for large ones during de novo formation of TJs, We also compared the RA-induced expression of TJ components and barrier function in RXR alpha (-/-)-RAR gamma (-/-) F9 cells with those in wild-type cells and show that the retinoid signals for transduction of these events are mediated by specific RXR-RAR pairs. (C) 2001 Academic Press.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available