Journal
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
Volume 1794, Issue 10, Pages 1421-1432Publisher
ELSEVIER
DOI: 10.1016/j.bbapap.2009.06.001
Keywords
Treponema denticola; Major protein; Antigen; Mass spectrometry
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Funding
- National Health and Medical Research Council Australia
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Treponema denticola is a Gram-negative, motile, asaccharolytic, anaerobic spirochaete which along with Porphyromonas gingivalis and Tannerella forsythia has been shown to form a bacterial consortium called the Red Complex that is strongly associated with the clinical progression of chronic periodontitis. T denticola was grown in continuous culture in a complex medium with a mean generation time of 15.75 h. Samples from two different membrane-enriched preparations and a cytoplasm-enriched preparation were separated by two-dimensional gel electrophoresis and the proteins identified by MALDI-TOF/TOF mass spectrometry. In total, 219 non-redundant proteins were identified including numerous virulence factors, lipoproteins, ABC transporter proteins and enzymes involved in the metabolism of nine different amino acids of which glycine seems to be of particular importance. Novel findings include the identification of several abundant peptide uptake systems, and the identification of three flagellar filament outer layer proteins. Two-dimensional Western blot analysis using sera from mice immunized with formalin-killed T denticola cells suggested that Msp, PrcA, OppA, OppA10. MgIB, TmpC and several flagellar filament proteins are antigenic. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.
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