Journal
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
Volume 1784, Issue 11, Pages 1590-1595Publisher
ELSEVIER
DOI: 10.1016/j.bbapap.2008.07.010
Keywords
Cytokinin; Mistletoe lectin; Zeatin; Microgravity; Plant hormone; Water stress
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Funding
- Deutsche Luft- und Raumfahrtagentur (DLR) [50WB0615]
- Polish Ministry of Higher Education and Science
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The crystal structure of mistletoe lectin I (ML-I) isolated from the European mistletoe Viscum album in complex with the most active phytohormone zeatin has been analyzed and refined to 2.54 angstrom resolution. X-ray suitable crystals of ML-I were obtained by the counter-diffusion method using the Gel-Tube R crystallization kit (GT-R) onboard the Russian Service Module on the international space station ISS. High quality hexagonal bipyramidal crystals were grown during 3 months under microgravity conditions. Selected crystals were soaked in a saturated solution of zeatin and subsequently diffraction data were collected applying synchrotron radiation. A distinct F-o-F-c electron density has been found inside a binding pocket located in subunit B of ML-I and has been interpreted as a single zeatin molecule. The structure was refined to investigate the zeatin-ML-I interactions in detail. The results demonstrate the ability of mistletoe to protect itself from the host transpiration regulation by absorbing the most active host plant hormones as part of a defense mechanism. (C) 2008 Elsevier B.V. All rights reserved.
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