Hsp90n-An accidental product of a fortuitous chromosomal translocation rather than a regular Hsp90 family member of human proteome

Human cells express two isoforms of the Hsp90 protein, called Hsp90(x and Hsp90 beta. Although existence of the third form called Hsp90 alpha Delta N, or Hsp90N was reported in 1998, our investigation, based on the sequence analysis and attempts to reproduce previous results, demonstrate that there is no evidence that Hsp90N gene is present in human genome and no homologs of such a protein are present in other known eukaryotic genomes. We propose that Hsp90N was created as an artifact of a cDNA synthesis or that it is a chimeric protein, being a result of the chromosomal rearrangement that occurred in a single cell line, after this line was established. (C) 2008 Elsevier B.V. All rights reserved.