Journal
MOLECULAR CELL
Volume 7, Issue 2, Pages 293-300Publisher
CELL PRESS
DOI: 10.1016/S1097-2765(01)00177-0
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- NIGMS NIH HHS [GM20056] Funding Source: Medline
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Saccharomyces cells with one unrepaired double-strand break (DSB) adapt after checkpoint-mediated G2/M arrest. Adaptation is accompanied by loss of Rad53p checkpoint kinase activity and Chkip phosphorylation. Rad53p kinase remains elevated in yku70 Delta and cdc5-ad cells that fail to adapt. Permanent G2/M arrest in cells with increased single-stranded DNA is suppressed by the rfa1-t11 mutation, but this RPA mutation does not suppress permanent arrest in cdc5-ad cells. Checkpoint kinase activation and inactivation can be followed in G2-arrested cells, but there is no kinase activation in G1-arrested cells. We conclude that activation of the checkpoint kinases in response to a single DNA break is cell cycle regulated and that adaptation is an active process by which these kinases are inactivated.
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