Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Volume 1833, Issue 3, Pages 559-572Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2012.11.020
Keywords
UCH37; PRP19; Migration; Invasion; HCC
Categories
Funding
- Ministry of Education
- Shanghai Science and Technology Commission [10410709400, 10411950100]
- National Nature Science Foundation of China [81000968, 81101540, 81101637, 81172273, 81272388]
- National Clinical Key Special Subject of China
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Ubiquitin C-terminal hydrolase 37 (UCH37) plays a crucial role in numerous biological processes and is also involved in oncogenesis. In this study, clinicopathologic data showed that UCH37 was over-expressed in hepatocellular carcinoma (HCC) cancerous tissues and was a significant predictor for time to recurrence (TTR). In vitro, we discovered that UCH37 could promote cell migration and invasion. Subsequently, we utilized Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to identify differentially expressed proteins in UCH37 over-expressing cells compared with the control cells, and found that PRP19, an essential RNA splicing factor, was up-regulated. The relationship between UCH37, PRP19 and the capability of cell migration and invasion was further confirmed. Collectively, this study demonstrated that UCH37 could promote cell migration and invasion in HCC cell lines through interacting and deubiquitinating PRP19, and suggested that UCH37 could be a novel predictor for HCC recurrence after curative resection. (C) 2012 Elsevier B.V. All rights reserved.
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