Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 20, Issue 5, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.20.5.057003
Keywords
blood plasma particles; dynamic light scattering; hydrodynamic radii; intensity size distribution; histogram of particle size registrations
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The work is devoted to the study of sizes and concentrations of proteins, and their aggregates in blood plasma samples, using static and dynamic light scattering methods. A new approach is proposed based on multiple repetition of measurements of intensity size distribution and on counting the number of registrations of different sizes, which made it possible to obtain statistically confident particle sizes and concentrations in the blood plasma. It was revealed that statistically confident particle sizes in the blood plasma were stable during 30 h of observations, whereas the concentrations of particles of different sizes varied as a result of redistribution of material between them owing to the protein degradation processes. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
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