Rapid high-performance liquid chromatography analysis for the quantitative determination of oleuropein in Olea europaea leaves

Olea europaea (Oleaceae) leaves of 14 different cultivars have been studied by a new isocratic HPLC method. Qualitative and quantitative determinations of principal compounds were established for each cultivar. Oleuropein concentration was determined for each sampled tree, using coumarin as internal standard. Bid el Haman, Chemlali, Meski, Cailletier, Tanche, a Verdale-Picholine hybrid, and Lucques, in particular, had high oleuropein concentrations and could be useful sources for industrial extractions.