Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Volume 1793, Issue 12, Pages 1894-1900Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2009.09.008
Keywords
Neutrophils; Resistin; Granules; Inflammation
Categories
Funding
- Iris scholarship
- Langman's culture fond
- Gamzeus foundation
- Medical Society of Gothenburg
- Swedish Association against Rheumatism
- King Gustaf V:s Foundation
- Swedish Medical Research Council
- Inflammation Network
- Nanna Swartz' Foundation
- AME Wolff Foundation
- Rune and Ulla Amlovs Trust
- Swedish Foundation for Strategic Research
- pharmacist Hedberg foundation
- University of Gothenburg
Ask authors/readers for more resources
We have recently shown that resistin is a key mediator of arthritis accumulating in the inflamed joints and exerting its pro-inflammatory properties independently of TNF alpha. Here we evaluate neutrophils as a cellular source of resistin. Human neutrophils were subjected to subcellular fractionation where the presence of resistin was assessed using western blot ELISA, and mass spectrometry. Presence of resistin on the neutrophil surface was visualized by flow cytometry. More than 95% of the neutrophils in circulation and in synovial fluid express resistin on their surface. Stimulation of mature neutrophils with fMLF induced release of resistin into supernatants and increased expression of resistin on the surface. Resistin is mobilized simultaneously with lactoferrin, a protein found in specific granules, and with granule-stored CR3/CD11b. Subcellular fractionation of human neutrophils demonstrated the presence of resistin in azurophilic and in specific granules. Here we show that neutrophils have two pools of resistin, the major one exists in specific granules, and the second on their cell membrane. Release of resistin from the neutrophil granules probably serves the main source of resistin at the site of inflammation. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available