Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Volume 1783, Issue 6, Pages 1111-1120Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2008.01.034
Keywords
Thy-1; integrins; brain cells; cell-cell interaction; adhesion molecules
Categories
Funding
- FIC NIH HHS [R03 TW007810, R03 TW006024-02, R03 TW006024-03, 1-R03-TW06024-03, R03 TW006024-01, R03 TW006024] Funding Source: Medline
- NIGMS NIH HHS [R01 GM029860, R01 GM029860-23, R01 GM029860-21, R01 GM029860-22] Funding Source: Medline
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Thy-1 is an abundant neuronal glycoprotein of poorly defined function. We recently provided evidence indicating that Thy-1 clusters a beta(3)-containing integrin in astrocytes to induce tyrosine phosphorylation, RhoA activation and the formation of focal adhesions and stress fibers. To date, the alpha subunit partner of beta(3) integrin in DI TNC1 astrocytes is unknown. Similarly, the ability of neuronal, membrane-bound Thy-1 to trigger astrocyte signaling via integrin engagement remains speculation. Here, evidence that alpha(V) forms an alpha(V)beta(3) heterodimer in DI TNC1 astrocytes was obtained. In neuron-astrocyte association assays, the presence of either anti-alpha(V) or anti-beta(3) integrin antibodies reduced cell-cell interaction demonstrating the requirement of both integrin subunits for this association. Moreover, anti-Thy-1 antibodies blocked stimulation of astrocytes by neurons but not the binding of these two cell types. Thus, neuron-astrocyte association involved binding between molecular components in addition to the Thy-1-integrin; however, the signaling events leading to focal adhesion formation in astrocytes depended exclusively on the latter interaction. Additionally, wild-type (RLD) but not mutated (RLE) Thy-1 was shown to directly interact with alpha(V)beta(3) integrin by Surface Plasmon Resonance analysis. This interaction was promoted by divalent cations and was species-independent. Together, these results demonstrate that the alpha(V)beta(3) integrin heterodimer interacts directly with Thy-1 present on neuronal cells to stimulate astrocytes. (C) 2008 Elsevier B.V. All rights reserved.
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