Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Volume 1864, Issue 11, Pages 3574-3584Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbadis.2018.08.022
Keywords
FTIR spectroscopy; IR imaging; Early micrometastasis; Pulmonary metastasis of breast cancer; Chemometrics
Funding
- Polish Ministry of Science and Higher Education [IP2015 048474]
- National Centre for Research and Development, Poland [METENDOPHA-STRATEGMED1/233226/NCBR/2015]
- National Science Centre, Poland [UMO 2016/23/B/NZ4/01379]
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An application of FTIR spectroscopic imaging for the identification and visualization of early micrometastasis from breast cancer to lungs in a murine model is shown. Spectroscopic and histological examination is focused on lung cross-sections derived from animals at the early phase of metastasis (early micrometastasis, EM) as compared to healthy control (HC) and late phase of metastasis (advanced macrometastasis, AM) using murine model of metastatic breast cancer with 4T1 cells orthotopically inoculated. FTIR imaging allows for a detailed, objective and label-free differentiation and visualization of EM foci including large and small micrometastases as well as single cancer cells grouped in clusters. An effect of the EM phase on the entire lung tissue matrix as well as characteristic biochemical profiles for HC and advanced macrometastasis were determined from morphological and spectroscopic points of view. The extraordinary sensitivity of FTIR imaging toward EM detection and discrimination of AM borders confirms its applicability as a complementary tool for the histopathological assessment of the metastatic cancer progression.
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