Journal
ANALYST
Volume 140, Issue 16, Pages 5619-5626Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an00736d
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Funding
- National Basic Research Program of China (973 Program) [2013CB933900]
- National Nature Science Foundation of China [21375097, 21175102, 21205090]
- Science Fund for Creative Research Groups of NSFC [20621502, 20921062]
- Fundamental Research Funds for the Central Universities [2015203020203]
- MOE of China
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Determination of trace elements in cells is critical to metallomics research and still faces tremendous difficulties even with the help of highly sensitive inductively coupled plasma mass spectrometry (ICP-MS). Microfluidic chips offer a functional tool-set for cell analysis with features of miniaturization, integration and automation. In this work, we proposed a chip-based array magnetic solid phase microextraction (MSPME) system and on-line combined it with ICP-MS via microflow concentric nebulization for the determination of trace Cu, Zn, Cd, Hg, Pb and Bi in cells. Under the optimized conditions, the limits of detection of the developed on-line chip-based array MSPME-ICP-MS system are 49, 43, 4.2, 6.1, 13 and 18 ng L-1 for Cu, Zn, Cd, Hg, Pb and Bi, respectively. The proposed method was applied to the analysis of target heavy metals in three kinds of cells (HepG2, Jurkat T and MCF7), and the recoveries for the spiked samples were in the range of 83.8%-117%. The extractable species of the target metals in cells were also studied and the results demonstrated a high selectivity of the developed methods towards free metal ions and/or their small molecular species.
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