Journal
BIOLOGICAL PSYCHIATRY
Volume 49, Issue 3, Pages 248-257Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0006-3223(00)01111-2
Keywords
amyloid; A beta peptide; nicotine; NMR; Alzheimer's disease
Categories
Funding
- NIA NIH HHS [AG-14363-04] Funding Source: Medline
Ask authors/readers for more resources
The major protein constituents of amyloid deposits in Alzheimer's disease (AD) are the 40-residue beta -amyloid (A beta) (1-40) peptide and the 42-residue A beta (1-42) peptide. The A beta (1-42) is more pathogenic and produced in greater quantities in familial forms of AD. A major goal of research is to uncover a suitable inhibitor that either slows down or inhibits A beta formation (beta -amyloidosis). During beta -amyloidosis, structural changes associated with the conversion of monomeric A beta peptide building blocks into the aggregated fibrillar beta -sheet structures occur (alpha -helix-->beta -sheet or random, extended chain-->beta -sheet). In previous work, we and others established that nicotine, a major component of cigarette smoke, inhibits beta -amyloidosis of the A beta (1-42), which may result from nicotine binding to the alpha -helical structure. These conclusions were based on solution nuclear magnetic resonance (NMR) spectroscopic studies with the nonnative 28-residue A beta (1-28). This information suggests that, when administered therapeutically to AD patients, nicotine may not only affect cholinergic activation, but could also conceivably alter amyloid deposition. In this report, NMR studies were augmented with the naturally occurring A beta (1-42), under conditions where the peptide folds into a predominantly alpha -helical or random, extended chain structure. The major result is that nicotine shows only modest binding to these conformations, indicating that the nicotine inhibition to beta -amyloidosis probably results from binding to a small, soluble beta -sheet aggregate that is NMR invisible. (C) 2001 Society of Biological Psychiatry.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available