Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Volume 1832, Issue 9, Pages 1456-1462Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbadis.2013.01.002
Keywords
Adiposity; Alzheimer's disease; Astrocyte; Diabetes; Inflammation; Microglia
Funding
- COBRE center from the National Institutes of Health [NIH 2P20-RR021945]
- NORC center from the National Institutes of Health, the Pennington Animal Metabolism and Behavior Core [NIH 2P30-DK072476]
- NIH
- Hibernia National Bank/Edward G Schleider Chair
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Cerebral amyloid angiopathy (CAA) occurs in nearly every individual with Alzheimer's disease (AD) and Down's syndrome, and is the second largest cause of intracerebral hemorrhage. Mouse models of CAA have demonstrated evidence for increased gliosis contributing to CAA pathology. Nearly two thirds of Americans are overweight or obese, with little known about the effects of obesity on the brain, although increasingly the vasculature appears to be a principle target of obesity effects on the brain. In the current study we describe for the first time whether diet induced obesity (DIO) modulates glial reactivity, amyloid levels, and inflammatory signaling in a mouse model of CAA. In these studies we identify surprisingly that DIO does not significantly increase A beta levels, astrocyte (GFAP) or microglial (IBA-1) gliosis in the CAA mice. However, within the hippocampal gyri a localized increase in reactive microglia were increased in the CA1 and stratum oriens relative to CAA mice on a control diet. DIO was observed to selectively increase IL-6 in CAA mice, with IL-1 beta and TNF-alpha not increased in CM mice in response to DIO. Taken together, these data show that prolonged DIO has only modest effects towards A beta in a mouse model of CAA, but appears to elevate some localized microglial reactivity within the hippocampal gyri and selective markers of inflammatory signaling. These data are consistent with the majority of the existing literature in other models of A beta pathology, which surprisingly show a mixed profile of DIO effects towards pathological processes in mouse models of neurodegenerative disease. The importance for considering the potential impact of ceiling effects in pathology within mouse models of A beta pathogenesis, and the current experimental limitations for DIO in mice to fully replicate metabolic dysfunction present in human obesity, are discussed. This article is part of a Special Issue entitled: Animal Models of Disease. (C) 2013 Published by Elsevier B.V.
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