Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 305, Issue 5, Pages 1025-1033Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1006/jmbi.2000.4362
Keywords
DNA oligonucleotides; counterion binding; capillary electrophoresis; free solution mobility; DNA bending
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Funding
- NIGMS NIH HHS [GM29690] Funding Source: Medline
- Telethon [E.0893] Funding Source: Medline
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The free solution mobility of four 20 bp DNA oligomers, with and without A-tracts, has been measured by capillary electrophoresis in Trisacetate buffer, to test the hypothesis that site-specific binding of monovalent counterions can occur in the narrow minor groove of A-tract DNAs. Preferential counterion binding has been proposed to cause A-tract bending because of asymmetric charge neutralization and collapse of the helix backbone toward the minor groove. Preferential counterion binding in A-tract DNAs should be manifested by a decrease in the electrophoretic mobility observed in free solution, compared to that of non-ii-tract DNAs of the same size. Of the four sequences studied here, the slowest absolute mobility, indicative of the greatest counterion binding, was observed for a 20 bp oligomer containing two runs of A(3)T(3) in phase with the helix repeat. A 20-mer containing phased CACA sequences migrated with the fastest mobility; 20-mers containing phased A(5) tracts or phased runs of T(3)A(3) migrated with intermediate mobilities. Very similar mobility differences were observed when 1-20 mM NaCl was added to the buffer. The results suggest that preferential counterion binding occurs in A-tract DNAs, especially those containing the A(n)T(n) sequence motif.
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