Journal
SCIENCE
Volume 291, Issue 5505, Pages 875-878Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.291.5505.875
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Funding
- NINDS NIH HHS [R01 NS36670-01A1] Funding Source: Medline
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Intracellular membrane fusion is mediated by the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins. All vesicle transport steps also have an essential requirement for a member of the Sec1 protein family, including the neuronal Munc18-1 (also known as nSec1) in regulated exocytosis. Here, in adrenal chromaffin cells, we expressed a Munc18 mutant with reduced affinity for syntaxin, which specifically modified the kinetics of single-granule exocytotic release events, consistent with an acceleration of fusion pore expansion, Thus, Munc18 functions in a late stage in the fusion process, where its dissociation from syntaxin determines the kinetics of postfusion events.
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