Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 751, Issue 1, Pages 61-68Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00450-3
Keywords
picolinic acid; Tryptophan
Funding
- Telethon [A.075] Funding Source: Medline
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A HPLC method is described to quantify picolinic acid in milk, blood serum and tissue culture supernatant. The method requires very little sample preparation because acid precipitation allows total recovery of picolinic acid. High specificity and sensitivity were obtained using ion-pair chromatography on a C-18 reversed-phase column with tetrabutylammonium hydrogen sulfate as ion pairing reagent. We describe the conditions for the automated testing of multiple samples and for the detection of L-tryptophan and L-kynurenine together with picolinic acid. This system will be utilized to elucidate the relationship between picolinic acid production and human disease. Furthermore, we provide the first evidence of picolinic acid in human blood serum. (C) 2001 Elsevier Science B.V. All rights reserved.
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