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JOURNAL OF IMMUNOLOGY
Volume 166, Issue 4, Pages 2427-2436Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.166.4.2427
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Biological activities of the matrix glycoprotein thrombospondin-1 (TSP1) are cell type specific and depend on the relative expression or activation of several TSP1 receptors, Although engaging individual TSP1 receptors in T lymphocytes can elicit costimulating signals, in this study we show that intact TSP1 inhibits TCR-mediated T cell activation, assessed globally using cDNA microarrays. TSP1 signaling suppressed expression of several genes induced in Jurkat T cells, including the T cell activation markers CD69, early growth response gene-1 (Egr-1), and phosphatase of activated cells (PAC-1), TCR-stimulated and CD47-costimulated IL-2 secretion and cell surface CD69 expression were also inhibited by TSP1. The specific inhibitory effect of TSP1 was verified in freshly isolated human PBMCs. TSP1 inhibited TCR-mediated but not protein kinase C-mediated T cell activation. Using CD69 expression as a marker, we demonstrated that the inhibitory activity of TSP1 depended on two TSP1 receptors, CD47 and integrin-associated protein heparan sulfate proteoglycans, Signals from these receptors inhibited TCR signaling downstream of ZAP70, but upstream of NF-AT, Therefore, the expression of TSP1 induced during wound repair and in tumor stroma may limit T cell activation at these sites. The Journal of Immunology, 2001, 166: 2427-2436.
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