Journal
NEUROSCIENCE LETTERS
Volume 299, Issue 1-2, Pages 65-68Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(00)01779-1
Keywords
excitatory postsynaptic current; glutamatergic; granule cell; mitral cell; oxytocin; olfactory bulb; primary culture
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Although oxytocin (OT) within the olfactory bulb has been implicated in maternal behaviour and olfactory recognition, the cellular mechanisms of action remain to be clarified. We examined the effects of OT on glutamatergic spontaneous excitatory postsynaptic currents (sEPSCs) in cultured granule cells with the use of whole-cell patch-clamp recordings. OT reversibly increased both the frequency and amplitude of sEPSCs. The effects of OT on sEPSCs were blocked by the selective OT receptor antagonists desGly-Nh(2)(9),d (CH2)(5)-[Thy(Me)(2),Thr(4)]-ornithine vasotocin. OT had no detectable effect, however, on high voltage-activated Ca2+ currents in mitral/tufted cells, suggesting that OT acts presynaptically on step(s) in the release process downstream from calcium influx. OT augmented the membrane current in granule cells evoked by exogenous application of glutamate, indicating a postsynaptic sire of action. These results indicate that OT facilitates sEPSCs in granule cells by both pre- and postsynaptic mechanisms. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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