Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Volume 1811, Issue 9, Pages 556-563Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2011.05.014
Keywords
Monocyte; THP-1 macrophage; Lipoprotein; Interleukin-1 receptor; Atherosclerosis
Funding
- Fundacio Privada Catalana de Nutricio i Lipids
- Spanish Pharmacology Society
- European Community
- Institut de Salud Carlos III (ISCIII) (Red de Enfermedades Cardiovasculares [RECAVA] [RD06/0014/0027, RD06/0014/0029]
- ISCIII
- Juan de la Cierva (MICINN)
- [2009SGR0413]
- [2010SAF-15664]
- [PS09/01797]
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Type II interleukin-1 receptor (IL-1R2) is a non-signaling decoy receptor that negatively regulates the activity of interleukin-1 (IL-1), a pro-inflammatory cytokine involved in atherogenesis. In this article we assessed the relevance of IL-1R2 in atherosclerosis by studying its expression in monocytes from hyperlipidemic patients, in THP-1 macrophages exposed to lipoproteins and in human atherosclerotic lesions. Our results showed that the mRNA and protein expression of IL-1R2 was reduced in monocytes from patients with familial combined hyperlipidemia (-30%, p<0.05). THP-1 macrophages incubated with increasing concentrations of acetylated low density (ac-LDL) and very low density (VLDL) lipoproteins also exhibit a decrease in IL-1R2 mRNA and protein levels. Pre-incubation with agents that block intracellular accumulation of lipids prevents the decrease in IL-1R2 mRNA caused by lipoproteins. Lipoproteins also prevented the increase in IL-1R1 and 1L-1R2 caused by a 4-h stimulation with LPS and reduced protein expression of total and phosphorylated IL-1 receptor-associated kinase-1. Finally, IL-1R2 expression in human atherosclerotic vessels was markedly lower than in non-atherosclerotic arteries (-80%, p<0.0005). Overall, our results suggest that under atherogenic conditions, there is a decrease in IL-1R2 expression in monocytes/macrophages and in the vascular wall that may facilitate IL-1 signaling. (C) 2011 Elsevier B.V. All rights reserved.
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