Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Volume 1811, Issue 12, Pages 1030-1037Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2011.08.007
Keywords
Phosphatidylethanolamine; Triacylglycerol; Acyltransferase; CDP-ethanolamine; Yeast; Saccharomyces cerevisiae
Funding
- Fonds zur Forderung der wissenschaftlichen Forschung in Osterreich [W901-B05, P21429]
- Austrian Science Fund (FWF) [P21429] Funding Source: Austrian Science Fund (FWF)
- Austrian Science Fund (FWF) [W 901, P 21429] Funding Source: researchfish
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In the yeast Saccharomyces cerevisiae triacylglycerols (TAG) are synthesized by the acyl-CoA dependent acyltransferases Dga1p, Are1p, Are2p and the acyl-CoA independent phospholipid:diacylglycerol acyltransferase (PDAT) Lro1p which uses phosphatidylethanolamine (PE) as a preferred acyl donor. In the present study we investigated a possible link between TAG and PE metabolism by analyzing the contribution of the four different PE biosynthetic pathways to TAG formation, namely de novo PE synthesis via Psd1p and Psd2p, the CDP-ethanolamine (CDP-Etn) pathway and lyso-PE acylation by Ale1p. In cells grown on the non-fermentable carbon source lactate supplemented with 5 mM ethanolamine (Etn) the CDP-Etn pathway contributed most to the cellular TAG level, whereas mutations in the other pathways displayed only minor effects. In cki1 Delta dpl1 Delta eki1 Delta mutants bearing defects in the CDP-Etn pathway both the cellular and the microsomal levels of PE were markedly decreased, whereas in other mutants of PE biosynthetic routes depletion of this aminoglycerophospholipid was less pronounced in microsomes. This observation is important because Lro1p similar to the enzymes of the CDP-Etn pathway is a component of the ER. We conclude from these results that in cki1 Delta dpl1 Delta eki1 Delta insufficient supply of PE to the PDAT Lro1p was a major reason for the strongly reduced TAG level. Moreover, we found that Lro1p activity was markedly decreased in cki1 Delta dpl1 Delta eki1 Delta, although transcription of LRO1 was not affected. Our findings imply that (i) TAG and PE syntheses in the yeast are tightly linked; and (ii) TAG formation by the PDAT Lro1p strongly depends on PE synthesis through the CDP-Etn pathway. Moreover, it is very likely that local availability of PE in microsomes is crucial for TAG synthesis through the Lro1p reaction. (C) 2011 Elsevier B.V. All rights reserved.
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