An epi-detected coherent anti-stokes raman scattering (E-CARS) microscope with high spectral resolution and high sensitivity

We report an epi-detected coherent anti-Stokes Raman scattering (E-CARS) microscope that uses two synchronized picosecond pulse trains. The microscope not only provides high spectral resolution but also significantly improves sensitivity for three-dimensional vibrational imaging of living cells.