Journal
JOURNAL OF CHROMATOGRAPHY B
Volume 751, Issue 2, Pages 297-303Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00488-6
Keywords
carotenoids
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We report a reversed-phase high-performance liquid chromatography method which resolves 13 identified carotenoids and nine unknown carotenoids from human plasma. A Nucleosil C-18 column and a Vydac C-18 column in series are used with an isocratic solvent system of acetonitrile-methanol containing 50 mM acetate ammonium-dichloromethane - water (70:15:10:5, v/v/v/v) as mobile phase at a flow-rate of 2 ml/min. The intra-day (4.5-8.3%) and inter-day (1.3-12.7%) coefficients of variation are suitable for routine clinical determinations. (C) 2001 Elsevier Science B.V. All rights reserved.
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